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Online QQ: Human Nicotinamide Adenine Dinucleotide Phosphate (NADPH) ELISA Kit
**Instructions for Use:**
1. Always prepare a standard curve during each test, and it is recommended to run duplicate wells for better accuracy. If the sample's concentration is too high (i.e., the OD value of the sample exceeds that of the first standard well), dilute the sample with an appropriate factor (n times) before testing. When calculating the results, multiply the measured value by both the dilution factor and the standard factor (×n×5).
2. The sealing film is designed for single-use only to prevent cross-contamination between different tests.
3. Store the substrate in a dark place to maintain its stability and performance.
**Additional Tips:**
- Ensure all reagents are brought to room temperature before use.
- Avoid touching the bottom of the microplate wells to prevent contamination.
- Follow the incubation time and temperature strictly as specified in the protocol.
- Read the absorbance at 450 nm using a microplate reader for accurate quantification.
- Properly dispose of all used materials according to local biohazard regulations.
- For any technical support or questions, feel free to contact us via phone or online chat.
This ELISA kit is ideal for measuring NADPH levels in biological samples such as serum, plasma, and cell lysates. It offers high sensitivity and specificity, making it suitable for research and diagnostic applications.
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