**Beijing Equation Biotelephone:** 010-82833221, 010-57266903, 13269288551
**Online QQ:** [Please check for contact details]
**Human Nicotinamide Adenine Dinucleotide Phosphate (NADPH) ELISA Kit**
**Instructions and Notes:**
1. **Standard Curve Preparation:** It is highly recommended to prepare a standard curve for every test run. Ideally, perform duplicate measurements for each standard point to ensure accuracy. If the sample's NADPH concentration is too high (i.e., the OD value of the sample exceeds that of the first standard well), it is advised to dilute the sample before testing. After obtaining the result, multiply the measured value by the dilution factor (e.g., ×n×5) to calculate the actual concentration in the original sample.
2. **Sealing Film Usage:** The sealing film is designed for single-use only. Reusing it may lead to cross-contamination between different samples, which could affect the reliability of your results. Always use a new sealing film for each plate.
3. **Substrate Handling:** The substrate solution is sensitive to light. Make sure to store it in a dark place and protect it from direct exposure during the entire procedure. This helps maintain its stability and ensures accurate color development during the reaction.
4. **Storage Conditions:** Store the kit components at 2–8°C when not in use. Avoid freezing unless specified on the product label. Always check the expiration date before starting the experiment.
5. **Quality Control:** To ensure consistent performance, it is recommended to include positive and negative controls in each assay. This helps verify the accuracy of the test and detect any potential issues early on.
6. **Washing Steps:** Proper washing is crucial for reducing background noise and improving the signal-to-noise ratio. Follow the washing instructions carefully, and ensure all wells are fully drained after each wash.
By following these guidelines, you can achieve more reliable and reproducible results with the Human NADPH ELISA Kit. For further assistance or technical support, feel free to contact us via phone or online.
Undermount Slides,undermount drawer slides,blum undermount drawer slides,bottom mount drawer slides,under mount drawer slides
Foshan Nanhai Ranming Lijiali Hardware Co., Ltd , https://www.lijialihardware.com