A customer who is new to ELISA experiments reached out to the manager at Shanghai Hengyuan Biological Company to place an order for a 96T human CSF kit. During their conversation, the customer had some questions about the difference between qualitative and quantitative ELISA methods. The company is known for its comprehensive pre-sales and after-sales support, including technical guidance throughout the experimental process. To help the customer better understand, Shanghai Hengyuan provided a clear explanation of how ELISA works for beginners:
ELISA, or Enzyme-Linked Immunosorbent Assay, is a laboratory technique used to detect and quantify specific substances in a sample by utilizing the highly specific interaction between antigens and antibodies. The intensity of the color produced during the reaction correlates with the concentration of the target analyte in the sample. There are two main types of ELISA: qualitative and quantitative.
Qualitative ELISA is used to determine whether a particular substance is present in the sample above or below a certain threshold. This type of test typically includes a positive control (P) and a negative control (N), and the results are reported as "positive" or "negative." The cut-off value, which is determined by the ELISA kit itself, is used to decide whether a result is considered positive or negative.
On the other hand, quantitative ELISA allows for more precise measurement. It involves creating a standard curve using a series of samples with known concentrations. By measuring the optical density (OD) values of these standards, a relationship between OD and concentration can be established. Once the standard curve is generated, the OD value of the unknown sample is plotted on the curve to determine its actual concentration. This method is widely used in research and diagnostic settings where accurate quantification is essential.
As a long-standing supplier of ELISA kits, biological reagents, antibodies, standards, and culture media, Shanghai Hengyuan Biological Company offers high-quality products at competitive prices. Their products are readily available and come with reliable quality assurance. Customers can easily place orders through phone, fax, email, or online. Whether you're a seasoned researcher or just starting out, the team at Shanghai Hengyuan is committed to providing excellent support and guidance to ensure your experiments are successful.
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