A first-time user of ELISA experiments reached out to the manager at Shanghai Hengyuan Biological Company to place an order for a 96T human CSF kit. During the conversation, the customer had some questions about the difference between qualitative and quantitative ELISA. Fortunately, Shanghai Hengyuan offers comprehensive pre-sales and after-sales support, including technical guidance throughout the experimental process. To help the customer better understand, the company explained the basics of ELISA for those new to the technique:
ELISA is a widely used laboratory method that detects the presence or quantity of specific substances in a sample by leveraging the specific binding between antigens and antibodies. The intensity of the color produced by the substrate correlates with the concentration of the target analyte in the sample. There are two main types of ELISA: qualitative and quantitative.
Qualitative ELISA is designed to determine whether a particular substance is present above or below a certain threshold. It typically includes a positive control (P) and a negative control (N), with results reported as either "positive" or "negative." The decision to classify a result as positive or negative is based on the cut-off value provided by the ELISA kit, which is determined during validation. This makes it useful for screening purposes but not for precise measurement.
On the other hand, quantitative ELISA provides more detailed information by generating a standard curve using a series of known concentrations. Each concentration corresponds to an optical density (OD) reading, allowing the sample's OD value to be compared against the curve to calculate its exact concentration. This type of ELISA is ideal when accurate measurements are required, such as in clinical or research settings.
As a trusted supplier of ELISA kits, biological reagents, antibodies, standards, and culture media, Shanghai Hengyuan has been specializing in importing high-quality scientific research materials for many years. We offer competitive pricing, fast delivery, and reliable quality assurance. Whether you prefer to place your order via phone, fax, email, or online, our team is here to assist you every step of the way.
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