Used for quantitative detection of mouse osteocalcin/glutamate protein (OT/BGP) levels in serum, plasma, and related fluid samples.
Mouse Osteocalcin/Bone Glutamate Protein (OT/BGP) Elisa Kit
English name: Mouse Osteocalcin/Bone gla protein, OT/BGP ELISA kit
Specifications: 96T/48T
Storage conditions: 2-8°C
ELISA kit sample processing and requirements:
1. Serum: Room temperature blood is allowed to clot for 10-20 minutes and centrifuged for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant and if sedimentation occurs during storage, centrifuge it again.
2. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifugation is performed for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant and if any precipitate forms during storage, it should be centrifuged again.
3. Urine: Collect with a sterile tube and centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again. Pleural fluid, cerebrospinal fluid reference implementation.
4. Cell culture supernatant: When detecting secretory components, use a sterile tube for collection. Centrifugation for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. When detecting intracellular components, the cell suspension was diluted with PBS (pH 7.2-7.4) to a cell concentration of about 1 million cells/ml. By repeated freezing and thawing, the cells are destroyed and the intracellular components are released. Centrifugation for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If precipitation forms during storage, it should be centrifuged again.
5. Organize the specimen: After cutting the specimen, weigh it. Add a certain amount of PBS, pH 7.4. Liquid nitrogen quickly frozen for use. After the sample melts, it still maintains a temperature of 2-8°C. Add a certain amount of PBS (PH7.4) and homogenize the sample by hand or homogenizer. Centrifugation for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. One part is to be tested after dispensing and the rest is frozen for use.
6. Samples should be extracted as soon as possible after collection. Extraction should be carried out according to the relevant literature. After the extraction, experiments should be carried out as soon as possible. If the test cannot be performed immediately, the specimen can be stored at -20°C, but repeated freezing and thawing should be avoided.
7. Samples containing NaN3 cannot be detected in ELISA kit experiments because NaN3 inhibits horseradish peroxidase (HRP) activity.